| Autor: |
Adriaens, Iris, Cortvrindt, Rita, Smitz, Johan |
| Přispěvatelé: |
Follicle Biology, Vrije Universiteit Brussel |
| Jazyk: |
angličtina |
| Rok vydání: |
2004 |
| Předmět: |
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| Popis: |
BACKGROUND: We investigated at what stage early cultured preantral mouse follicles become dependent on a minimal effective FSH dose (10 mIU/ml) and analysed the influence of implementing FSH at several time-points during in vitro culture. METHODS: Two-layered mouse follicles were cultured for 12 days under seven different FSH exposure regimens and ovulated on day 13 by hCG/EGF. Ovulated cumulus-oocyte complexes were fertilized and embryos were cultured up to the blastocyst stage. RESULTS: When FSH was absent or added only once at the start of culture, follicle survival was significantly reduced (22 and 52% respectively versus 95% when FSH was continuously present, P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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