| Autor: |
Bertelli, P.r., Rico, Eduardo P., Grünspan, Lauren D., Biegelmeyer, Renata, Klein Junior, Luiz Carlos, Yvan, Vander Heyden, Gasper, André L., Bordignon, Sérgio A.L., Oliveira, Diogo L., Henriques, Amélia T. |
| Přispěvatelé: |
Analytical Chemistry and Pharmaceutical Technology, Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Popis: |
The Psychotria L. genus is chemically characterized by the presence of indole alkaloids. Our research group has already demonstrated that these alkaloids are able to inhibit enzymes in vitro, such as cholinesterases and monoamine oxidases. The fish embryo acute toxicity (FET) test is an important dose-screening tool: it is a rapid, simple and effective method. This screening established by the OECD 236 (Organization for Economic Co-operation and Development) was used to define the best dose that can be use in the future studies to evaluate the biochemical parameters in the nervous system of adults Zebrafish. In addition, a preliminary chemical evaluation of enriched alkaloids fraction (EFA) obtained from P. deflexaleaves is also objective of this study. The leaves of P. deflexa were collected in the Rain Forest at Blumenau (SC-Brazil). The air-dried material was powdered and macerated with EtOH. After solvent evaporation, the crude extract was submitted to acid-base partition to obtain the EAF. This fraction was analyzed by HPLC-DAD and High-resolution Mass Spectrometry (HRMS) to determine their chemical profiles. In addition, the FET evaluation of the EAF (1; 5; 10; 20; 30; 40; 50; 75 e 100 μg/mL) was performed. The following parameters were evaluated: coagulation of fertilized eggs; lack of somite formation; lack of detachment of the tail-bud from the yolk sac; and lack of heartbeat. The HPLC-DAD analysis showed the presence of several monoterpene indole alkaloids in the EAF. HRMS analysis identified the major product having a molecular ion m/z = 517.2188 (C26H32N2O9) as strictosidinic acid. The FET test demonstrated that the EAF (1-50 ug/ml) did not produce morphological changes after 96 hpf. However, higher concentrations resulted in coagulation of embryos at the period of 48 hpf. From these results it was possible to establish an appropriate dose for the study of biochemical parameters in zebrafish central nervous system such as cholinergic activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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