Development of DGT sampler for monitoring estrogenic activity in water with ERE-CALUX bioassay
| Autor: | Guo, Wei, Van Langenhove, Kersten, Elskens, Marc, Gao, Yue |
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| Přispěvatelé: | Chemistry, Analytical, Environmental & Geo-Chemistry, Earth System Sciences, Faculty of Sciences and Bioengineering Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
interregional input–output model
Counterpart assistance Global and Planetary Change natural disaster Sociology and Political Science Environmental Science(all) internal and external validity Geography Planning and Development indirect economic losses development hormones hormone substitutes and hormone antagonists resilience and recovery |
| Popis: | Monitoring the estrogenic effects of these compounds demands efficient, simple and sensitive techniques. In this study, a combination of a new type of diffusive gradients in thin-film (DGT) technique with an estrogen responsive element-chemically activated luciferase gene expression (ERE-CALUX) bioassay was developed. The performance of this novel method was assessed with 17β-estradiol (E2) as the model steroid hormone, XAD 18 resin gel as binding phase in the DGT method and VM7Luc4E2 cells (formerly BG1Luc4E2) for the ERE-CALUX bioassay. The laboratory tests showed that DGT components and the experimental matrix do not influence the estrogenic activity of ERE-CALUX cells. The effective diffusion coefficient of steroid hormones in agarose diffusive gel was 5.16 ×10-6 cm2s-1 at 25 °C. The detection limit of this combined DGT/ERE-CALUX method for a 1 day sampling period (0.026 ng L-1of E2), is significantly lower than that obtained by active sampling combined with GC-MS/MS and LC-MS/MS analysis. This method wasindependent of pH (5-8),ionic strength (0.001-0.5 M) and dissolved organic matter (DOM) concentrations(0-30 mg L-1). The estrogenic effects of steroid hormones of effluent in Beijing sewage treatment plant determined by DGT for a 6h deployment time were comparable to those measured by an active sampling method, within a relative standard deviation of 20%. This study demonstrates that DGT combined with ERE-CALUX is an effective tool for pre-concentrating steroid estrogens and possibly also in the monitoring of estrogenic activity in wastewaters. |
| Databáze: | OpenAIRE |
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