| Autor: |
Laureys, G, Valentino, M, Demol, F, Zammit, C, Muscat, R, Cambron, M, Kooijman, R, De Keyser, J |
| Přispěvatelé: |
Basic (bio-) Medical Sciences, Experimental Pharmacology, Neuroendocrine Immunology, Faculty of Medicine and Pharmacy, Neuroprotection & Neuromodulation, Clinical sciences |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
In vitro studies have demonstrated that β2-adrenergic receptor activation stimulates glycogen degradation in astrocytes, generating lactate as a potential energy source for neurons. Using in vivo microdialysis in mouse cerebellar white matter we demonstrate continuous axonal lactate uptake and glial-axonal metabolic coupling of glutamate/lactate exchange. However, this physiological lactate production was not influenced by activation (clenbuterol) or blocking (ICI 118551) of β2-adrenergic receptors. In two-photon imaging experiments on ex vivo mouse corpus callosum subjected to aglycemia, β2-adrenergic activation rescued axons, whereas inhibition of axonal lactate uptake by α-cyano-4-hydroxycinnamic acid (4-CIN) was associated with severe axonal loss. Our results suggest that axonal protective effects of glial β2-adrenergic receptor activation are not mediated by enhanced lactate production. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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