| Autor: |
Demaegdt, Heidi, De Backer, Jean-Paul, Lukaszuk, Aneta, Tóth, Géza, Szemenyei, E, Tourwe, Dirk, Vauquelin, Georges |
| Přispěvatelé: |
Molecular and Biochemical Pharmacology, Chemistry, Department of Bio-engineering Sciences, Organic Chemistry |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Popis: |
Radioligand binding studies revealed that Ang IV binds to IRAP/'AT4 receptors' with high affinity. Yet, as these experiments were routinely done in the presence of chelators, only the catalytic zinc-depleted apo-form of IRAP was labelled. While the chelators remove the catalytic zinc from IRAP and protect Ang IV from proteolytic degradation, the Aminopeptidase N selective inhibitor '7B' only exerts the latter effect. By using 7B along with the new stable Ang IV-analog [3H]AL-11, we here show that the native enzyme is only a low affinity target for Ang IV. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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