| Autor: |
Čemažar, Maja, Grošel, Alenka, Kranjc, Simona, Pipan Tkalec, Živa, Serša, Gregor |
| Jazyk: |
angličtina |
| Rok vydání: |
2003 |
| Zdroj: |
Radiology and oncology (Ljubljana) |
| Popis: |
Aim. Radiosensitization with cisplatin can be enhanced by electroporation of cells and tumours. The aim of this study was to extend our previous studies ontwo carcinoma tumour models with different chemo-and radiosensitivity in order to evaluate whether this treatment is effective also on less chemo-and radiosensitive tumour cells. Materials and methods. This in vitro study was performed on carcinoma SCK and EAT-E cells. The cytotoxicity of three-modalitytreatment consisting of cisplatin, electroporation and irradiation was determined by the clonogenic assay. Results. The radiosensitizing effect of cisplatin on the two cell lines was greatly enhanced by electroporation. By this combined treatment, less chemo and radiosensitive EAT-E cells were rendered as sensitive as more chemo and radiosensitive SCK cells. Conclusion. The enhancement of cisplatin-induced radiosensitization of cells by electroporation could be beneficially used in the treatment of intrinsically less chemo- and radiosensitive tumours. Izhodišča. Z elektroporacijo celic in tumorjev lahko povečamo njihovo občutljivost za cisplatin. Namen raziskave je bil razširiti našo predhodno raziskavo na dva tumorska modela karcinoma z različno občutljivostjo za kemoterapijo in obsevanje, ter ugotoviti, ali je takšno zdravljenje učinkovitotudi pri celicah, ki so manj občutljive za kemoterapijo in obsevanje. Materiali in metode. V in vitro raziskavi smo uporabili celični liniji karcinoma SCK in EAT-E. Citotoksično delovanje kombiniranega zdravljenja s cisplatinom, elektroporacijo in obsevanjem smo določili s testomklonogenosti. Rezultati. Z elektroporacijo se je radiosenzibilizirajoči učinek cisplatina na testiranih celičnih linijah zelo povečal. Manj kemo- in radio-občutljive celice EAT-E so po kombiniranem zdravljenju postale enako občutljive kot celice SCK, ki so bolj občutljive za kemoterapijo in obsevanje.Zaključki. Povečano občutljivost celic za cisplatin po elektroporaciji lahko koristno uporabimo tudi za zdravljenje manj občutljivih celic za kemoterapijo in obsevanje. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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