Molecular analysis of the goat b-lactoglobulin gene: preliminary results
Autor: | PAPPALARDO, MARGHERITA, GALLO, DANIELA, COSENZA, GIANFRANCO, RAMUNNO, LUIGI, PASTORE N., SENESE C., RUBINO R., MARLETTA D. |
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Přispěvatelé: | Pappalardo, Margherita, Gallo, Daniela, Cosenza, Gianfranco, Pastore, N., Senese, C., Rubino, R., Marletta, D., Ramunno, Luigi |
Jazyk: | angličtina |
Rok vydání: | 2001 |
Popis: | Analysis of the goat beta-lactoglobulin gene evidenced three differences, two in the 7th exon (a G--A transition in position 83 and a 10 nucleotides, 5'-AGGCCCCTCC-3', insertion/deletion rearrangement phenomenon located downstream nucleotide 104) and one in the 6th intron (an A--G transition located 7 nucleotides upstream the 7th exon). In order to identify carriers of the two exonic mutations, a PCR-RFLP method of analysis has been set up. The frequency of the insertion in a local goat population reared in province of Naples and in Girgentana goats reared in Basilicata and Sicilia was 0.09 and 0.01 respectively |
Databáze: | OpenAIRE |
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