Expression of the RET/PTC fusion gene as a marker for papillary carcinoma in Hashimoto's thyroiditis
| Autor: | A. Wirtschafter, R. Schmidt, D. Rosen, N. Kundu, H. Multhaupt, J. P. Atkins, M. R. Rosen, W. M. Keane, J. L. Rothstein, SANTORO, MASSIMO, FUSCO, ALFREDO |
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| Přispěvatelé: | A., Wirtschafter, R., Schmidt, D., Rosen, N., Kundu, Santoro, Massimo, Fusco, Alfredo, H., Multhaupt, J. P., Atkin, M. R., Rosen, W. M., Keane, J. L., Rothstein |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
methods
Proto-Oncogene Proteins c-ret Proto-Oncogene Protein genetics RNA Papillary Messenger complications/genetics Thyroiditi Biological Adult Aged Carcinoma complications/genetics Tumor Marker complications/genetics DNA Primers Drosophila Proteins Female Gene Expression Humans Male Middle Aged Oncogene Protein genetics/isolation /&/ purification RNA-Directed DNA Polymerase Receptor Protein-Tyrosine Kinase genetics Polymerase Chain Reaction genetics Thyroid Neoplasm genetics Autoimmune |
| Popis: | Hashimoto's thyroiditis is an inflammatory disease of the thyroid gland with autoimmune etiology. Patients afflicted with Hashimoto's have a higher risk of thyroid malignancies such as papillary thyroid carcinoma. In the present study, we investigated the frequency of papillary thyroid carcinoma specific genes in patients diagnosed with Hashimoto's disease. The newly identified oncogenes RET/PTC1 and RET/PTC3 provide useful and specific markers of the early stages of papillary carcinoma as they are highly specific for malignant cells. Using a sensitive and specific reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we found messenger RNA (mRNA) expression for the RET/PTC1 and RET/PTC3 oncogenes in 95\% of the Hashimoto's patients studied. All Hashimoto's patients presenting without histopathologic evidence of papillary thyroid cancer showed molecular genetic evidence of cancer. These data suggest that multiple, independent occult tumors exist in these patients at high frequency. |
| Databáze: | OpenAIRE |
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