| Autor: |
D'ADDONA, ALESSIO, SOMMA, DOMENICO, FORMISANO, SILVESTRO, ROSA, DONATO, DI DONATO, SIGISMONDO LUCA, MAFFULLI, NICOLA |
| Přispěvatelé: |
D'Addona, Alessio, Somma, Domenico, Formisano, Silvestro, Rosa, Donato, DI DONATO, SIGISMONDO LUCA, Maffulli, Nicola |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Popis: |
The aim of our study is to investigate the behaviour of healthy and tendinopathic human tenocytes after a heat shock. After we harvested tendinopathic and healthy human tendon samples, we split tenocytes into 4 groups: 3 groups were submitted to heat shock, followed by different periods of post-heating (2, 4 and 20 h). The other group represents our negative control. The target genes were analysed using Real Time PCR. IL-1β and IL-6 expression were significantly increased in tendinopathic samples after heat shock. COL1 and COL3 expression were increased in non-stimulated tendinopathic tenocytes, but their levels significantly decreased after heat shock (p less than 0.01). COL3 levels increase in healthy samples after 20 h post-heating (p less than 0.01). COL1 and COL3 decreased after heat shock as a sign of the failure of repair mechanisms in tendinopathic tendons. Heat shock in in vitro models was insufficient to trigger pro-inflammatory cytokines in healthy human tenocytes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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