Linear aminolipids with moderate antimicrobial activity from the antarctic gram-negative bacterium aequorivita sp
| Autor: | Chianese G., Esposito F. P., Parrot D., Ingham C., de Pascale D., Tasdemir D. |
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| Přispěvatelé: | Chianese, G., Esposito, F. P., Parrot, D., Ingham, C., de Pascale, D., Tasdemir, D. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Antarctic Region
Molecular Structure Methicillin-Resistant Staphylococcus aureu Microbial Sensitivity Test Metabolomic MRSA Sequence Analysis DNA Chemical Fractionation Aequorivita Gram-negative bacterium Amino Acid Geologic Sediment Miniaturized culture chip Anti-Bacterial Agent Spectroscopy Fourier Transform Infrared Seawater LC-MS/MS Linear aminolipid Flavobacteriaceae Fatty Acid |
| Popis: | The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1⁻3) and four known (4⁻7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C-3 hydroxy groups. The chemical structures of the new compounds were elucidated using a set of spectroscopic (NMR, [α]D and FT-IR) and spectrometric (HRMS, HRMS/MS) methods. The aminolipids possessing an N-terminal glycine unit (1, 2, 4, 5) showed moderate in vitro antimicrobial activity against MRSA (IC50 values 22⁻145 μg/mL). This is the first in-depth chemistry and biological activity study performed on the microbial genus Aequorivita. |
| Databáze: | OpenAIRE |
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