| Autor: |
COCCIA E. M., VAIMAN D., RABER J., MARZIALI G., FIORUCCI G., ORSATTI R., COHEN B., NISSIM N., ROMEO G., CHEBATH J. AND BATTISTINI A., AFFABRIS, Elisabetta |
| Přispěvatelé: |
Coccia, E. M., Vaiman, D., Raber, J., Marziali, G., Fiorucci, G., Orsatti, R., Cohen, B., Nissim, N., Romeo, G., Affabris, Elisabetta, Chebath, J. AND BATTISTINI A. |
| Jazyk: |
angličtina |
| Rok vydání: |
1991 |
| Předmět: |
|
| Popis: |
The induction of transcription of the 2'-5'-oligoadenylate (2-5A) synthetase gene by type I (alpha/beta) and type II (gamma) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-alpha/beta addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-gamma, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-alpha/beta-resistant FLC, IFN-gamma induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-gamma-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding enzymatic activity in both w.t. and IFN-alpha/beta-resistant FLC. These findings suggest that F1 and Fg represent two distinct effector complexes by which type I and type II IFNs, respectively, induce 2-5A synthetase. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
