| Přispěvatelé: |
Nardini, R., Scicluna, M., Terregino, C., Mandola, M., Cavaliere, N., Cordioli, P., Fusco, G., Purpari, G., Angioini, A., Panzieri, C., Manca, M., Autorino, G. |
| Popis: |
Data on evaluation of some diagnostic parameters of all ELISA kits available in Italy for the serodiagnosis of AIE are presented and discussed. Ten laboratories were involved using a panel of 30 sera ran with 4 commercial kits and 2 in-house kits. Kits were also evaluated using a panel of sera from vaccinated animals at different days post vaccination (p.v.). All sera were also tested in agar gel immunodiffusion (AGID). The parameters evaluated were: diagnostic sensitivity (DSe) and specificity (DSp), Cohen K, weighted Cohen K, coefficient of variation (CV), accordance, concordance. Analysis of these parameters indicates that all kits have a higher sensitivity than AGID, even if a complete evaluation, as indicated by OIE (1) should be carried out. |
