| Autor: |
GIACALONE, Antonio Mario, MONTALTO, Giuseppe, GIANNITRAPANI, Lydia, BALASUS, Daniele, SORESI, Maurizio, Cervello, M, Marasà, L. |
| Přispěvatelé: |
Giacalone, A, Montalto, G, Giannitrapani, L, Balasus, D, Cervello, M, Soresi, M, Marasà, L. |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
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| Popis: |
Introduction: TNF-a, COX-2 and Vascular Endothelial Growth Factor (VEGFA) are mediators of inflammation and angiogenesis, all of them are abundantly produced in liver cirrhosis (LC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and hepatocellular carcinoma (HCC). These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several anti-angiogenic agents have entered clinical trials in HCC. Aims: 1) To evaluate thè frequency of SNPs of COX-2, TNF-a and VEGF-A genes in patients with HCC vs LC patients and a control group (C). 2) To verify thè correlation between thè allelic variations and the risk of developing HCC tumors. Methods: DNA extracted from whole blood of C, HCC and LC patients (all from Sicily, Italy), was used for the evaluation of SNPs by the RFLP-PCR method. The SNPs analyzed were at position -1195 G>A in the promoter regions, of COX-2 (A allele is associated with higher levels of thè enzyme), at position -308 G>A in thè promoter regions of TNF-a genes (G allele is associated with lower levels of the cytokine), and at position +936 C>T in the coding region of VEGFA gene (T allele is associated with high levels of the growth factor). X2, Fisher exact tests were used for statistical analysis. Results: We obtained a significant difference only for the SNP +936 C>7 of VEGF-A gene. Results obtained are shown in the following table. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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