| Autor: |
Prawirosukarto, Sudharto, Pardede, D., Veyrunes, J.C., Cousserans, François, Philippe, René, Desmier De Chenon, Roch, Mariau, Dominique, Bergoin, M. |
| Jazyk: |
angličtina |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
International conference on Technology transfer in biological control : de la recherche à la pratique, Montpellier, 9-11 septembre 1996. Résumés |
| Popis: |
Among of the oil palm leaf-eating nettle caterpillars in Indonesia, Setothosea asigna Van Eecke is the most important species, attacking indifferently the young and the old plantations. We have recently isolated from infected-dead larvae of this defoliator collected in North Sumatra a small RNA virus that we characterized as belonging to the #Nudaurelia-beta# group. This virus proved to be highly pathogenic for #S. asigna# larvae in laboratory conditions. In order to develop a method for the biological control of this pest based on the spraying of this virus in the oil palm plantations in Indonesia, we have prepared appropriate diagnosis tools i.e. : polyclonal antibodies against purified virions for ELISA tests and a cDNA probe for dot-blot analysis. Both diagnostic methods proved to be efficient for detecting the presence of the virus in infected caterpillars. The sensitivity of larvae according to the instar and to virus concentration was determined in laboratory conditions. Field applications of viral preparations were performed and the comparative results of larval population dynamics in treated and untreated plots will be presented. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
