The diversity of insect and plant trypanosomatids: molecular (PCR) and morphology data

Autor: Podlipaev, Sergei A., Frolov, A., Bulat, S., Banuls, A.L., Dollet, Michel
Jazyk: francouzština
Rok vydání: 1998
Předmět:
Zdroj: The flagellates : International Symposium of the Systematics Association, Birmingham, September 7th-10th, 1998
Popis: The most unreliable part in the taxonomic system of the trypanosomatids is the domain of trypanosomatids from insects and plants. The relationships within insect trypanosomatids are still obscure. Twenty-two isolates from insects (both reference strains and recent isolates) and four isolates from plants were, investigated by RAPD, UP-PCR and cross dot-blot hybridization of UP-PCR products. Ultrastructure and some additional characters (polymorphism of colonies on solid medium, DNA content by flow cytometry etc.) were also examined. It was concluded that: I. The main clusters on dendrograms derived from PCR are supported by ultrastructural data; 2. Leptomonas-like isolates are very heterogeneous by all methods used thus confirming the view of Leptomonas as an artificial group; 3. The number of current genera of trypanosomatids is not enough to demonstrate the real diversity of these parasites and new genera have to be established.
Databáze: OpenAIRE