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Cybridisation is a plant biotechnology tool, which can be used to improve the resistanc of citrus rootstocks to salinity. To validade this approach, cuttings of six diploid cybrids obtained by symmetric fusion as well as their parents were used: Willow leaf mandarin (Citrus deliciosa) + (C. macrophylla), Willow leaf mandarin (Citrus deliciosa) + Citrumelo 4475 (C. paradisi x P. trifoliata), Willow leaf mandarin (Citrus deliciosa Ten.) + Rangpur lime (C. limonia), F (Willow lead mandarin (Citrus deliciosa) + Rangpur lime (C. limonia)), Willow leaf mandarin (Citrus deliciosa) + C35 (C. sinensis x P. trifoliata), Willow leaf mandarin (Citrus deliciosa) + Citrandarin (C. reticula x P. trifoliata) and Poncirus trifoliata. The plants were subjected to different doses of NaCl: 0, 50, 100 and 150 mM. The evaluation of salinity effects on growth parameters and the comparison of Cl accumulation among the genotypes studied led us to conclude that all these parameters were affected. However, cybrids seemed to be tolerant, sensitive or "moderately tolerant" compared to the parental genotypes. Rangpur lime cybrids showed particularly a differential response to salinity. When the cybrid F expressed an impressive resistance, it was found that the other cybrid RL behaves in a similar way to that of the parents. (Texte intégral) |
