Assessment of Phytophthora pod rot resistance by means of attached pod inoculation

Autor: Luz, Edna Dora Martin Newman, Silva, Setal D.V.M., Blaha, Georges
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: Working procedures for cocoa germplasm evaluation and selection : Proceedings of the CFC/ICCO/IPGRI Project workshop, 1-6 February 1998, Montpellier, France
Popis: To evaluate local and international accessions with a view to comparison of resistance to black pod disease employing a method which reflects intrinsic pod resistance. The advantage of using point-inoculation with Phytophthora spp. zoospore suspension of unwounded attached pods is that it makes it possible to determine both types of resistance to penetration (infection frequency) and to colonization (lesion size). The inoculum quantification is precise and the inoculum is fully standardized. With this method, development of the lesion can be followed for a sufficiently long period. Measurements taken only once for lesion size mean that this screening test can be applied to large quantities of test materials. Another important aspect is that resistance is evaluated "in vivo" and "in loco". Results from this test have shown correspondence with the responses of natural infection in the field (Pires et al. 1994; Luz et al. 1999).
Databáze: OpenAIRE