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Tema ovog rada je mikroinkapsulacija probiotičkog soja Lactobacillus fermentum D12 u alginatu uz dodatak prebiotika inulina i fruktooligosaharida, te liofilizacija mikroinkapsuliranih stanica uz dodatak obranog mlijeka kao lioprotektora. Cilj rada bio je utvrditi štite li prebiotici i obrano mlijeko mikroinkapsulirane stanice tijekom procesa sušenja, skladištenja i izlaganja stanica nepovoljnim uvjetima gastrointestinalnog trakta. Vrlo je važno da se stanice probiotičkih sojeva zaštite na odgovarajući način kako bi se što veći broj tih stanica dostavio do ciljnog mjesta kao što je gastrointestinalni trakt i kako bi tamo započele svoje korisno djelovanje. Određivanjem preživljavanja stanica mikroinkapsuliranih uz dodatak prebiotika i liofiliziranih uz dodatak lioprotektora, nakon procesa liofilizacije i nakon izlaganja stanica nepovoljnim uvjetima gastrointestinalnog trakta i usporedbom preživljavanja u odnosu na kontrolu, utvrđeno je kako alginat kao sredstvo za mikroinkapsulaciju i dodatak prebiotika i lioprotektora štite stanice od nepovoljnih i stresnih uvjeta. The topic of this study is the microencapsulation of the Lactobacillus fermentum D12 probiotic strain in alginate with the addition of prebiotics (inuline and fructo-oligosaccharide), and the lyophilization of microencapsulated cells with the addition of skimmed milk as a lyoprotectant. The aim of the study was to determine whether will prebiotics and skim milk protect microencapsulated cells during the process of drying, storage and exposing cells to unfavourable conditions of the gastrointestinal tract. It is very important that probiotic cells are adequately protected so that as many of these cells are delivered to the target site in the gastrointestinal tract and to begin their beneficial effects. Determination of survival of the microencapsulated cells with addition of prebiotics and lyophilized with addition of lyoprotectant after the lyophilization process and post-exposure of the cells to unfavorable conditions of the gastrointestinal tract and comparison of survival to the control sample, showed that the alginate as a microencapsulating medium and addition of prebiotics and lyoprotectant protect cells from adverse and stressful conditions. |
