| Popis: |
OBJECTIVE: To examine the effects of age and/or diabetes on oxidative stress and steroidogenesis, and the protective effect of vitamin E in testis tissue. DESIGN: Controlled experimental study. SETTING: Pamukkale University School of Medicine animal facility. ANIMAL(S): Male Wistar rats divided into six groups with six animals in each group: young control; young diabetic; young diabetic with vitamin E treatment; aged control; aged diabetic; and aged diabetic with vitamin E treatment. INTERVENTION(S): Diabetes was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin and was confirmed by testing blood glucose levels 5 to 7 days after injection. Vitamin E was administered orally for 6 weeks. MAIN OUTCOME MEASURE(S): Serum testosterone and tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) levels were measured, and testis tissue was examined histopathologically. RESULT(S): Elevated malondialdehyde and reduced superoxide dismutase, glutathione, and serum testosterone levels were detected only in the young and aged-diabetic groups. Histopathologic change was not detected in the testis tissue in any of the groups. CONCLUSION(S): Age does not alter the effects of diabetes-induced free radical damage in testis tissue; improvement in this damage can be achieved by vitamin E treatment. |
