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Uvod: Karies je najpogostejša zobna bolezen , ki nastane zaradi demineralizacije trdih zobnih tkiv. Bakterija Streptococcus mutans je glavni povzročitelj kariesa, saj s presnovo ogljikovih hidratov proizvaja kislino, ki poruši pH ravnovesje v ustni votlini. Saharoza velja za najbolj kariogen ogljikov hidrat, ki povzroča tvorbo biofilmov in spodbuja adhezijo bakterij. Robovi zobnih nadomestkov predstavljajo idealna mesta za adhezijo bakterij, kar lahko povzroči bolezni kot so sekundarni karies, parodontitis in periimplantitis. Namen: Namen diplomskega dela je ugotoviti, kako različne koncentracije saharoze vplivajo na adhezijo S. mutans na površini Co-Cr in kompozita. Cilj diplomskega dela je ugotoviti pri kateri koncentraciji saharoze je adhezija največja. Metode dela: V praktičnem delu diplomskega dela smo pripravili vzorce površin Co-Cr in kompozita. Tem površinam smo izmerili fizikalne lastnosti. Izmerili smo hrapavost, mejni kot in zeta potencial. V mikrobiološkem laboratoriju smo pripravili čisto kulturo S. mutans in gojišča z različnimi koncentracijami saharoze. Za določanje adhezije S. mutans smo uporabili dve metodi. Pokritost smo določali z vrstičnim elektronskim mikroskopom, s pomočjo mikrografov, absorbanco pa smo merili s spektofotometrom. V zobnem laboratoriju smo naredili fasetirane prevleke z ogrodjem iz Co-Cr in kompozitno faseto. Rezultati: Pri kompozitu smo izmerili višjo hrapavost (^^= 0,32 µm), kot pri Co-Cr (^^=0,21 µm). Povprečni mejni kot površine Co-Cr je (76,66 ± 2,39)°, kompozita pa (74,16 ± 2,95)°, kar pomeni, da sta obe površini hidrofilni. Povprečna vrednost zeta potenciala pri površini Co-Cr je -52,428 mV, pri kompozitu pa -25,912 mV. Obe površini sta negativno nabiti. Pri SEM metodi smo najvišjo stopnjo adhezije S. mutans na Co-Cr in kompozit določili pri 5 % saharoze, najnižjo pa pri 0 % saharoze. Pri metodi barvanja s kristal vijoličnim barvilom smo najvišjo stopnjo adhezije S. mutans na Co-Cr in kompozit določili pri 2,5 %, najnižjo pa pri 0 %. Razprava in zaključek: Rezultati naše študije kažejo, da ima saharoza vpliv na adhezijo S. mutans na površino Co-Cr in kompozita. Adhezija se je večala z višanjem deleža saharoze. Hrapavost, hidrofobnost in zeta potencial vplivajo na adhezijo S. mutans. Introduction: Dental caries is the most common dental disease, caused by demineralisation of enamel. The bacteria Streptococcus mutans is the main cause od dental caries, because it produces acid by metabolising carbohydrates, which disrupts the pH of the oral cavity. Sucrose is considered to be the most cariogenic carbohydrate, causing biofilm porosity and promoting bacterial adhesion. The edges of dental restorations are ideal sites for bacterial adhesion, which can lead to diseases such as secondary caries, parodontitis and periimplantitis. Purpose: The purpose of the bachelor's thesis is to determine how different concentrations of sucrose affect the adhesion of S. mutans on Co-Cr and composite. The aim is to determine at which sucrose concentration, the adhesion is the highest. Methods: In the practical part of the bachelor's thesis, samples of Co-Cr and composite surfaces were prepared. The physical properties of these surfaces were measured. We measured the roughness, hydrophobicity and zeta potential. We prepared a pure cuture of S. mutans and media with different concentrations of sucrose in the microbiology laboratory. Adhesion was measured by SEM method and crystal violet staining. In the dental laboratory, we made a crown with a Co-Cr framework anf a composite veneer. Results: Higher roughness was measured for the composite with ^^ of (0.32 ± 0.06) μm and lower roughness for Co-Cr with ^^ of (0.21 ± 0.03) μm. The superficial limiting angle of the Co-Cr surface is (76,66 ± 2,39) ° and that of the composite is (74,16 ± 2,95)°, indicating that both surfaces are hydrophilic. The average value of the zeta potential for the Co-Cr surface is (-52,428 ± 0,322) mV and for the composite is (-25,912 ± 10,09) mV. Both surfaces are negatively charged. In the SEM method, the highest level of adhesion of S. mutans to Co-Cr and the composite was determined at 5% sucrose and the lowest at 0% sucrose. In the crystal violet dye method, the highest level of adhesion of S. mutans to Co-Cr and the composite was determined at 2,5 % and the lowest at 0 %. Discussion and conclusion: The results of our study show that sucrose has an effect on the adhesion of S. mutans to the surface of Co-Cr and the composite. The adhesion increased with increasing sucrose content. Comparing the individual sucrose ratios, we find that the composite has a higher degree of S. mutans adhesion than Co-Cr. The roughness, hydrophobicity and zeta potential affect the adhesion of S. mutans. |
