| Autor: |
Proj, Matic, Knez, Damijan, Sosič, Izidor, Gobec, Stanislav |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Drug discovery today, vol. 27, no. 6, pp. 1733-1742, 2022. |
| ISSN: |
1359-6446 |
| Popis: |
Compounds that exhibit assay interference or undesirable mechanisms of bioactivity are routinely encountered in assays at various stages of drug discovery. We observed that assays for the investigation of thiol-reactive and redox-active compounds have not been collected in a comprehensive review. Here, we review these assays and subject them to experimental optimization to improve their reliability. We demonstrate the usefulness of our assay cascade by assaying a library of bioactive compounds, chemical probes, and a set of approved drugs. These high-throughput assays should complement the array of wet-lab and in silico assays during the initial stages of hit discovery campaigns to pursue only hit compounds with tractable mechanisms of action. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect