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'Candidatus Phytoplasma solani' je bakterija brez celične stene, ki je obligatni parazit rastlin. Je povzročiteljica bolezni na številnih rastlinah na vinski trti na primer povzroča navadno trsno rumenico. S sekvenciranjem produktov ugnezdene verižne reakcije s polimerazo (nPCR) genov tuf, secY in stamp smo določili molekulsko raznolikost sevov 'Ca. P. solani' izoliranih iz vinske trte. Z analizo njihovega časovnega in geografskega pojavljanja v Sloveniji ter primerjavo rezultatov z rezultati podobnih analiz v Evropi, še posebej v okoliških državah, smo določili možno pot širjenja navadne trsne rumenice med Slovenijo in okoliškimi državami. 'Candidatus Phytoplasma solani' is a bacteria without a cell wall, which is an obligatory parasite of plants. It causes diseases in many plants, for example: Bois Noir on grapevine. By sequencing nested polymerase chain reaction products of tuf, secY and stamp genes, we determined the molecular diversity of 'Ca. P. solani' strains isolated from grapevines. By analyzing their occurrence in Slovenia on a geographical and time scale and then comparing the results with similar analysis in Europe, especially in surrounding countries, we determined possible pathways by which spreading of Bois Noir grapevine disease, between Slovenia and its neighbouring countries, can occur. |
