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Adhezini so beljakovinske površinske strukture bakterij, ki se specifično vežejo na receptorje gostiteljske celice in na pritrjevalne površine. Najpogostejši receptorji oz. tarčne molekule so različni glikoproteini in saharidni ostanki na površini celic gostitelja. Namen raziskave je primerjava različnih najpogostejših adhezinov patogenih bakterij rodu Salmonella, Escherichia in Campylobacter. Med njimi so najpogostejši fimbrijski adhezini, ki so tudi ključni za ireverzibilno pritrditev na površino. Bakterije rodu Campylobacter nimajo fimbrij oz. jih pri njih še niso odkrili, zato primerjava teh ni bila mogoča. Podobnosti adhezinov bakterij pa smo ugotovili pri vezavi na tarčne molekule kot so fibronektin, laminin in manoza. S to ugotovitvijo lahko sklepamo, da mehanizem vezave adhezinov lahko predstavlja novo potencialno strategijo nadzora nad patogenimi bakterijami, saj lahko tarčne molekule zamenjamo z različnimi učinkovinami. Poleg tega pa smo ugotovili, da imajo vsi trije rodovi bakterij izraženo flagelo, ki pripomore k adheziji na površino. Adhezini tako predstavljajo specifičen odtis vsakega rodu bakterij. Adhesins are protein cell surface components or appendages of bacteria that enable adhesion to host cell receptors and adhesion surfaces. The most common targets (receptors) are various glycoproteins and sugar residues such as the mannose receptor. The aim of this work was to compare different adhesins between pathogenic bacteria Salmonella, Escherichia and Campylobacter. Fimbrial adhesins are the most abundand and play the major role in ireversible adhesion to the surface. Bacteria of the genus Campylobacter do not have fimbriae (they have not yet been discovered), so the comparison between them was not possible. The similarity between adhesins that adhere to target molecules such as fibronectin, laminin and mannose was discovered. We concluded that adhesins represent a new potential control strategy to interfere with pathogenic bacteria. The target molecules can be replaced by different substances. Furthermore, we discovered that all the mentioned bacteria have expressed flagella, which contributes to adhesion. All adhesins represent a specific expression of individual bacteria. |
