Delination of catheter-associated factors of urinary tract infections in vitro
| Autor: | Rošker, Patrik |
|---|---|
| Přispěvatelé: | Mlinarič-Raščan, Irena |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
udc:579:616-022.1(043.3)
P. mirabilis biofilms Catheter-associated urinary tract infection (CAUTI) in vitro dynamic catheterized bladder model two-component regulatory pathway system TargeTron® mutagenesis urinske infekcije dvo-komponentni regulatorni sistem TargetZron mutageneza vstavljen urinski kateter model mehurja |
| Popis: | Background and aim: One of the most common pathogens responsible for urinary tract infections, especially in patients undergoing long-term catheterization, is Gram-negative Proteus mirabilis. It leads to biofilm formation on catheter surface, encrustation and blockage of urinary catheter and contributes to persistence of catheter-associated urinary tract infections (CAUTI). This study aims to provide new insights into genetic factors of P. mirabilis relevant for CAUTI by elaborating importance of ompR, cpxA, arcA and arcB genes, in a dynamic catheterized bladder model for in vitro mimicking of physicochemical conditions in a catheterized human bladder. Methods: For evaluation of ompR, cpxA, arcA and arcB sgenes, we attempted to generate knockout mutants by using site-specific TargeTron® mutagenesis. Growth characteristics of successfully generated HI4320arcB mutant were evaluated with separate and co-challenge test. Relative gene expression of selected genes in wild-type (WT) were monitored by RT-qPCR using ΔΔCt method. Additionally, the importance of arcA and arcB in anaerobic conditions and cpxA and ompR in hight osmolarity environment was elaborated by using P12H3(arcA), P17- E5(cpxA), P70C10(ompR), transposon and HI4320arcB knockout mutant in media containing higher concentrations of urea, NaCl and H2O2. Results and conclusions: Single strain test revealed that there appears to be no difference in growth rate (p>0.7) between WT and HI4320arcB mutant. During co-challenge test competitive index (CI) indicated reduced growth of HI4320arcB in catheter [0.16] and in bladder [0.12]. Wilcoxon test confirmed these findings (P>0.05). HI4320arcB was significantly (P0.05, p= 0.686), medtem ko smo v mehurju in na katetru 24 ur po infekciji dokazali, da je divji tip v rasti signifikantno premagal HI4320arcB mutanto (P |
| Databáze: | OpenAIRE |
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