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This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia, an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan’s blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages. Fil: Guerra, Miguel P.. Universidade Federal de Santa Catarina; Brasil Fil: Steiner, Neusa. Universidade Federal de Santa Catarina; Brasil Fil: Farias Soares, Francine L.. Universidade Federal de Santa Catarina; Brasil Fil: Vieira, Leila do N.. Universidade Federal de Santa Catarina; Brasil Fil: Fraga, Hugo P. F.. Universidade Federal de Santa Catarina; Brasil Fil: Rogge Renner, Gladys D.. Universidade Federal de Santa Catarina; Brasil. University of Joinville Region; Brasil Fil: Maldonado, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina |
