Evaluation of total white blood cell count as a marker for proviral load of bovine leukemiavirus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus

Autor: Alvarez, Irene, Gutierrez, Geronimo, Gammella, Mariela, Martínez, Cecilia Magalí, Politzki, Romina, Gonzalez, Cintia Daniela, Caviglia, Luciana, Carignano, Hugo Adrian, Fondevila, Norberto Antonio, Poli, Mario Andres, Trono, Karina Gabriela
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Popis: Objective: To determine the normal reference interval for white blood cell (WBC) counts in dairy Holstein cows in Argentina and to analyze the correlation of total WBC counts and blood proviral load (PVL) in Bovine leukemia virus (BLV)-infected animals, with the final purpose to identify a hematological marker that can estimate the level of in vivo infection. Animals ? A total of 307 lactating cows from 16 dairy herds with high BLV seroprevalence. Procedure ? Blood samples were used for manual determination of WBC count, BLV p24 antibodies by ELISA, and PVL by real-time PCR. Results ? The normal reference interval of WBC for BLV-negative dairy cows was stated as 2153-11493 cells/ul. In the herd used to analyze the correlation between WBC count and PVL, 73% of the animals showed normal values of WBC counts. Of them, only 19.6% showed high PVL. Most of the animals with high WBC counts (35/39) showed high PVL. The mean WBC count was significantly higher among the animals with high PVL than among those with low or undetectable PVL (P
Databáze: OpenAIRE