| Autor: |
Höfig, I., Ehrhardt, H., Jeremias, I. |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Zdroj: |
Cell Commun. Signal. 10:8 (2012) |
| Popis: |
Background: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice. Results: We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations. Conclusion: Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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