Distribution of sphingosine kinase activity and mRNA in rodent brain

Autor: Blondeau N., Lai Y., Tyndall S., Popolo M., Topalkara K., Pru J.K., Zhang L., Kim H., Liao J.K., Ding K., Waeber C.
Přispěvatelé: Blondeau, N., Institut de Pharmacologie Moléculaire et Cellulaire (IPMC), UMR 6097, CNRS-UNSA, 660 route des lucioles, Sophia Antipolis, 06560 Valbonne, France, Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States -- Lai, Y., Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States -- Tyndall, S., Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States -- Popolo, M., Service de Neurologie, Hôpital Pasteur, Av de la voie Romaine, 06002 Nice Cedex 1, France, Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States -- Topalkara, K., Department of Neurology, Faculty of Medicine, Cumhuriyet University, Sivas, 58140, Turkey, Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States -- Pru, J.K., Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Charlestown, MA, United States -- Zhang, L., Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Charlestown, MA, United States -- Kim, H., Vascular Medicine Research, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, United States -- Liao, J.K., Vascular Medicine Research, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, United States -- Ding, K., Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States -- Waeber, C., Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, United States, Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, United States
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Popis: Sphingosine-1-phosphate (S1P) is a lipid mediator that exerts multiple cellular functions through activation of a subfamily of G-protein-coupled receptors. Although there is evidence that S1P plays a role in the developing and adult CNS, little is known about the ability of brain parenchyma to synthesize this lipid. We have therefore analyzed the brain distribution of the enzymatic activity of the S1P synthesizing enzyme, sphingosine kinase (SPHK) [EC:2.7.1.91], as well as mRNA distribution for one of the two isoforms of this enzyme, sphingosine kinase 2. SPHK activity, measured by the conversion of [3H]sphingosine to [3H]S1P, is highest in cerebellum, followed by cortex and brainstem. Lowest activities were found in striatum and hippocampus. Sensitivity to 0.1% Triton-X suggests that this activity is accounted for by SPHK2. RT-PCR and in situ hybridization studies show that mRNA for this isoform has a distribution similar to that of SPHK activity. In vivo and in vitro ischemia increase SPHK activity and SPHK2 mRNA levels. These results indicate that SPHK2 is the predominant S1P-synthesizing isoform in normal brain parenchyma. Its heterogeneous distribution, in particular laminar distribution in cortex, suggests a neuronal localization and a possible role in cortical and cerebellar functions, in normal as well as ischemic brain. © 2007 The Authors.
Databáze: OpenAIRE