Determination of Urinary Total Hydroxyproline by HPLC with UV and EC Detectors
| Autor: | Kaya, Betul Isiner, Dural, Emrah, Kenduzler, Erdal, Soylemezoglu, Tulin |
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| Přispěvatelé: | [Kaya, Betul Isiner -- Kenduzler, Erdal] Burdur Mehmet Akif Ersoy Univ, Fac Arts & Sci, Dept Chem, TR-15030 Burdur, Turkey -- [Dural, Emrah] Sivas Cumhuriyet Univ, Fac Pharm, Dept Pharmaceut Toxicol, TR-58140 Sivas, Turkey -- [Soylemezoglu, Tulin] Ankara Univ, Inst Forens Sci, Dept Forens Chem & Forens Toxicol, TR-06590 Ankara, Turkey |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Popis: | WOS: 000444687300005 Hydroxyproline is found in high concentrations in connective tissue proteins. It is remarkably useful to detect it because variation in urine levels of hydroxyproline is associated with various diseases. High performance liquid chromatography methods, in which ultraviolet and electrochemical detectors were used, were developed and validated for the determination of hydroxyproline in urine. Both methods included acid hydrolysis and derivation. The most appropriate time, temperature and pH were identified to optimize derivatization processes. Limits of detection were calculated as 1.57 mu g/mL, 0.9 mu g/mL and limits of quantification were 4.76 mu g/mL, 2.73 mu g/mL for UVD and ECD methods, respectively. Precision and accuracy of the methods were obtained Scientific Research Projects Support Program of Burdur Mehmet Akif Ersoy University [0146-YL-11] This research was supported by Scientific Research Projects Support Program of Burdur Mehmet Akif Ersoy University under the project number of 0146-YL-11. |
| Databáze: | OpenAIRE |
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