Effects of gallic acid on expressions of MMP-2 and MMP-9 through the pathway of p38/JNK in C6 glioma cells
| Autor: | GÜLSOY, NAGİHAN |
|---|---|
| Přispěvatelé: | Yaka E. B., Yazici Z. M. C., Ersoz M., GÜLSOY N. |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Farmakoloji
INVASION Life Sciences (LIFE) PROTEIN Pharmacy oxidative damage Sağlık Bilimleri BREAST ANGIOGENESIS Drug Guides glioma Yaşam Bilimleri Health Sciences FARMAKOLOJİ VE ECZACILIK Farmakoloji Toksikoloji ve Eczacılık (çeşitli) Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics CANCER-CELLS Eczacılık PHARMACOLOGY & PHARMACY Pharmacology Toxicology and Pharmaceutics (miscellaneous) Pharmacology PHARMACOLOGY & TOXICOLOGY SP600125 Temel Bilimler Basic Pharmaceutics Sciences PROLIFERATION Life Sciences toxicity INHIBITOR Pharmacology and Therapeutics Genel Farmakoloji Toksikoloji ve Eczacılık VIABILITY Farmakoloji (tıbbi) Matrix metalloproteinase Temel Eczacılık Bilimleri İlaç Rehberleri Yaşam Bilimleri (LIFE) Farmakoloji ve Toksikoloji gallic acid JNK Natural Sciences |
| Popis: | In our study, the effects of gallic acid (GA), a natural therapeutic agent, on oxidative stress biomarkers and MMP-2 and MMP-9 expressions via the p38/JNK pathway in C6 glioma cells were investigated. The toxicity of GA was determined by the WST-1 method. JNK, p38 and MMP-2/-9 mRNA expressions in the cell line were detected by RT-qPCR. JNK/SAPK, Grap-2/p38 and MMP-2/-9 protein levels were analyzed by using ELISA methods. Biochemical markers were analyzed. GA reduced the cell viability of C6 glioma cells after 24, 48 and 72h of treatment. The expression levels of MMP-2 and MMP-9 mRNA decreased in C6 glioma cells treated with 150 mu g/ml GA for 24 and 48h compared to the control cells. Unlike SOD activity, GA treatment significantly increased PCO and MDA levels in the cells treated with 150 mu g/ml GA for 24 and 48h compared to the non-treated cells. According to our results, GA inhibited the proliferation of C6 glioma cells. Also, it reduced MMP-2 and MMP-9 expressions and increase oxidative stress. Therefore, GA may have preventive effects on gliomas progression and/or invasion. |
| Databáze: | OpenAIRE |
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