Morphological and biochemical investigation of the protective effects of panax ginseng on methotrexate-induced testicular damage

Autor: AKBAY, TUĞBA, ERCAN, FERİHA
Přispěvatelé: Karakaya F. B., Macit Ç., Sivas G. G., Akbay T., Şeber G., Ercan F.
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Temel Tıp Bilimleri
Histoloji-Embriyoloji
Life Sciences (LIFE)
Medicine (miscellaneous)
CELL BIOLOGY
Assessment and Diagnosis
testis
Sağlık Bilimleri
Temel Bilgi ve Beceriler
Genel Tıp
Fundamental Medical Sciences
Pathophysiology
Clinical Medicine (MED)
TIP
GENEL & DAHİLİ
Health Sciences
Yaşam Bilimleri
Internal Medicine
oxidative stress
Klinik Tıp (MED)
Aile Sağlığı
MEDICINE
GENERAL & INTERNAL
Moleküler Biyoloji ve Genetik
Dahiliye
Patofizyoloji
Klinik Tıp
Hücre Biyolojisi
Histology and Embryology
Fundamentals and Skills
Panax ginseng
Life Sciences
General Medicine
CLINICAL MEDICINE
HÜCRE BİYOLOJİSİ
Değerlendirme ve Teşhis
Tıp
MOLECULAR BIOLOGY & GENETICS
Methotrexate
Yaşam Bilimleri (LIFE)
General Health Professions
Medicine
Tıp (çeşitli)
Family Practice
Genel Sağlık Meslekleri
Popis: Objective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types ofcancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX.Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose ofMTXdissolved in saline(20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis sampleswere obtained. Seminiferous tubules and basement membranewere evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured.Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen’s score, the seminiferous tubule’sdiameter, and the germinal epithelium’s thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity.Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-inducedtesticular damage by inhibiting oxidative stress.
Databáze: OpenAIRE
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