RNA synthesis inhibition stabilises urokinase mRNA in macrophages
| Autor: | Stacey, K J, Nagamine, Y, Hume, D A |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Mice
Inbred BALB C Amanitins Base Sequence Macrophage Colony-Stimulating Factor Macrophages Molecular Sequence Data Cell Differentiation Urokinase-Type Plasminogen Activator Recombinant Proteins Kinetics Mice Oligodeoxyribonucleotides Bone Marrow Enzyme Induction Dactinomycin Animals Humans RNA RNA Messenger Dichlororibofuranosylbenzimidazole |
| Zdroj: | Stacey, K J, Nagamine, Y & Hume, D A 1994, ' RNA synthesis inhibition stabilises urokinase mRNA in macrophages ', FEBS Letters, vol. 356, no. 2-3, pp. 311-3 . |
| Popis: | Urokinase-type plasminogen activator (uPA) mRNA is induced in macrophages by the lineage specific growth factor CSF-1. Upon removal of CSF-1 from bone marrow-derived macrophages (BMM), uPA mRNA decayed with a half-life of 2 h. If RNA synthesis inhibitors actinomycin D, 5,6-dichloro-1-beta-ribofuranosyl benzimidazole (DRB) or alpha-amanitin were added at the time as CSF-1 removal, the uPA message was stabilised. This was not a general effect on CSF-1 responsive mRNAs, as c-myc mRNA decayed with normal kinetics in the presence of inhibitors. The requirement for ongoing RNA synthesis for the degradation of uPA mRNA in BMM suggests that a component of the degradative pathway may be induced following removal of CSF-1. |
| Databáze: | OpenAIRE |
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