Core alpha 1 -> 3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep

4(Fuc alpha 1 -->3)GlcNAc beta 1-Asn (core alpha 1-->3-Fuc) and Xyl beta 1-2Man beta 1 --> 4GlcNAc beta 1-R (core beta 1 --> 2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha 1-->3-Fuc epitope, Evidence is presented that core alpha 1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H, contortus infected sheep. These results suggest that the core alpha 1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE, In addition we show here that IV-glycans carrying core alpha 1-->3-Fuc and beta 1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha 1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope, (C) 1999 Federation of European Biochemical Societies. -->
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Jazyk: English
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=od______3094::ea651c89ecc8da5a58788ba459a0317c
https://hdl.handle.net/20.500.11820/52264458-b868-47a3-be28-d52663298202
Rights: OPEN
Přírůstkové číslo: edsair.od......3094..ea651c89ecc8da5a58788ba459a0317c
Autor: van Die, Irma, Gomord, [No Value], Kooyman, FNJ, van den Berg, TK, Cummings, RD, Vervelde, L
Jazyk: angličtina
Rok vydání: 1999
Předmět:
Zdroj: van Die, I, Gomord, N V, Kooyman, FNJ, van den Berg, TK, Cummings, RD & Vervelde, L 1999, ' Core alpha 1-> 3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep ', FEBS Letters, vol. 463, no. 1-2, pp. 189-193 .
Popis: Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain R-GlcNAc beta 1-->4(Fuc alpha 1 -->3)GlcNAc beta 1-Asn (core alpha 1-->3-Fuc) and Xyl beta 1-2Man beta 1 --> 4GlcNAc beta 1-R (core beta 1 --> 2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha 1-->3-Fuc epitope, Evidence is presented that core alpha 1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H, contortus infected sheep. These results suggest that the core alpha 1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE, In addition we show here that IV-glycans carrying core alpha 1-->3-Fuc and beta 1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha 1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope, (C) 1999 Federation of European Biochemical Societies.
Databáze: OpenAIRE
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