Development of a high-speed line-scanning FLIM microscope for biological imaging
| Autor: | Mai, Hanning, Jarman, Anneliese, Erdogan, Ahmet, Treacy, Conor, Finlayson, Neil, Henderson, Robert K., Poland, Simon |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Zdroj: | Mai, H, Jarman, A, Erdogan, A, Treacy, C, Finlayson, N, Henderson, R K & Poland, S 2023, ' Development of a high-speed line-scanning FLIM microscope for biological imaging ', Optics Letters, vol. 48, no. 8, pp. 2042-2045 . https://doi.org/10.1364/OL.482403 |
| Popis: | We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications. |
| Databáze: | OpenAIRE |
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