The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state
| Autor: | Lowe, Kate L, Navarro-Nuñez, Leyre, Bénézech, Cécile, Nayar, Saba, Kingston, Bethany L, Nieswandt, Bernhard, Barone, Francesca, Watson, Steve P, Buckley, Christopher D, Desanti, Guillaume E |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Zdroj: | Lowe, K L, Navarro-Nuñez, L, Bénézech, C, Nayar, S, Kingston, B L, Nieswandt, B, Barone, F, Watson, S P, Buckley, C D & Desanti, G E 2015, ' The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state ', European Journal of Immunology, vol. 45, no. 9 . https://doi.org/10.1002/eji.201445314 |
| DOI: | 10.1002/eji.201445314 |
| Popis: | Expression of mouse CLEC-2 has been reported on circulating CD11b(high) Gr1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC-2 expression failed to use CLEC-2-deficient mice as negative controls and instead relying heavily on single antibody clones. Here, we generated CLEC-2-deficient adult mice using two independent approaches and employed two anti-mouse CLEC-2 antibody clones to investigate surface expression on haematopoietic cells from peripheral blood and secondary lymphoid organs (SLOs). We rule out constitutive CLEC-2 expression on resting DCs and show that CLEC-2 is up-regulated in response to LPS-induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC-2 and suggest that both circulating B lymphocytes and CD11b(high) Gr1(high) myeloid cells lose CLEC-2 following entry into SLOs. These results have significant implications for our understanding of CLEC-2 physiological functions This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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