| Autor: |
Van Nues, Robert Willem, Granneman, Sander, Kudla, Grzegorz, Sloan, Katherine Elizabeth, Chicken, Matthew, Tollervey, David, Watkins, Nicholas James |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Van Nues, R W, Granneman, S, Kudla, G, Sloan, K E, Chicken, M, Tollervey, D & Watkins, N J 2011, ' Box C/D snoRNP catalysed methylation is aided by additional pre-rRNA base-pairing ', EMBO Journal, vol. 30, no. 12, pp. 2420-2430 . https://doi.org/10.1038/emboj.2011.148 |
| Popis: |
2'-O-methylation of eukaryotic ribosomal RNA (r)RNA, essential for ribosome function, is catalysed by box C/D small nucleolar (sno)RNPs. The RNA components of these complexes (snoRNAs) contain one or two guide sequences, which, through base-pairing, select the rRNA modification site. Adjacent to the guide sequences are protein-binding sites (the C/D or C'/D' motifs). Analysis of >2000 yeast box C/D snoRNAs identified additional conserved sequences in many snoRNAs that are complementary to regions adjacent to the rRNA methylation site. This 'extra base-pairing' was also found in many human box C/D snoRNAs and can stimulate methylation by up to five-fold. Sequence analysis, combined with RNA-protein crosslinking in Saccharomyces cerevisiae, identified highly divergent box C'/D' motifs that are bound by snoRNP proteins. In vivo rRNA methylation assays showed these to be active. Our data suggest roles for non-catalytic subunits (Nop56 and Nop58) in rRNA binding and support an asymmetric model for box C/D snoRNP organization. The study provides novel insights into the extent of the snoRNA-rRNA interactions required for efficient methylation and the structural organization of the snoRNPs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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