Osteoclast nuclei of myeloma patients show chromosome translocations specific for the myeloma cell clone: a new type of cancer-host partnership?:a new type of cancer-host partnership?
| Autor: | Levin Andersen, Thomas, Boissy, Patrice, Sondergaard, T E, Kupisiewicz, Katarzyna, Plesner, T, Rasmussen, T, Haaber, J, Kølvraa, S, Delaissé, Jean-Marie |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
musculoskeletal diseases
Male Integrins Acid Phosphatase Osteoclasts Hybrid Cells Translocation Genetic hemic and lymphatic diseases Image Interpretation Computer-Assisted In Situ Nick-End Labeling Humans Receptors Vitronectin Interphase In Situ Hybridization Fluorescence Aged Cell Nucleus Cell Differentiation Middle Aged Flow Cytometry Coculture Techniques Clone Cells Isoenzymes Bromodeoxyuridine Microscopy Fluorescence Tumor Markers Biological Female Syndecan-1 Multiple Myeloma |
| Zdroj: | Levin Andersen, T, Boissy, P, Sondergaard, T E, Kupisiewicz, K, Plesner, T, Rasmussen, T, Haaber, J, Kølvraa, S & Delaissé, J-M 2007, ' Osteoclast nuclei of myeloma patients show chromosome translocations specific for the myeloma cell clone: a new type of cancer-host partnership? a new type of cancer-host partnership? ', Journal of Pathology, vol. 211, no. 1, pp. 10-17 . https://doi.org/10.1002/path.2078 |
| Popis: | Udgivelsesdato: 2007-Jan A major clinical manifestation of bone cancers is bone destruction. It is widely accepted that this destruction is not caused by the malignant cells themselves, but by osteoclasts, multinucleated cells of monocytic origin that are considered to be the only cells able to degrade bone. The present study demonstrates that bone-resorbing osteoclasts from myeloma patients contain nuclei with translocated chromosomes of myeloma B-cell clone origin, in addition to nuclei without these translocations, by using combined FISH and immunohistochemistry on bone sections. These nuclei of malignant origin are transcriptionally active and appear fully integrated amongst the other nuclei. The contribution of malignant nuclei to the osteoclast population analysed in this study was greater than 30%. Osteoclast-myeloma clone hybrids contained more nuclei than normal osteoclasts and their occurrence correlated with the proximity of myeloma cells. Similar hybrid cells were generated in myeloma cell-osteoclast co-cultures, as revealed by tracing myeloma nuclei using translocations, bromo-deoxyuridine, or the Y chromosome of male myeloma cells in female osteoclasts. These observations indicate that hybrid cells can originate through fusion between myeloma cells and osteoclasts. In conclusion, malignant cells contribute significantly to the formation of bone-resorbing osteoclasts in multiple myeloma. Osteoclast-myeloma clone hybrids reflect a previously unrecognized mechanism of bone destruction in which malignant cells participate directly. The possibility that malignant cells corrupt host cells by the transfer of malignant DNA may have been underestimated to date in cancer research. |
| Databáze: | OpenAIRE |
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