Ageing is associated with diminished muscle re-growth and myogenic precursor cell expansion early after immobility-induced atrophy in human skeletal muscle
| Autor: | Suetta, Charlotte Arneboe, Frandsen, Ulrik, Mackey, Abigail L, Jensen, Line, Hvid, Lars G, Beyer, Monika L, Petersson, Stine J, Schrøder, Henrik D, Andersen, Jesper L, Aagaard, Per, Schjerling, Peter, Kjaer, Michael |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Muscular Atrophy/physiopathology Cyclin-Dependent Kinase Inhibitor p27/genetics Receptor Fibroblast Growth Factor Type 1/genetics Cyclin-Dependent Kinase Inhibitor p21/genetics RNA Messenger/metabolism Middle Aged Tumor Suppressor Proteins/genetics Aging/physiology Muscle Skeletal/physiology Myoblasts/physiology Young Adult STAT5 Transcription Factor/genetics Proto-Oncogene Proteins c-met/genetics Humans Myostatin/genetics Female PAX7 Transcription Factor/genetics Immobilization/physiology Insulin-Like Growth Factor I/genetics MyoD Protein/genetics Aged Hepatocyte Growth Factor/genetics |
| Zdroj: | Suetta, C A, Frandsen, U, Mackey, A L, Jensen, L, Hvid, L G, Beyer, M L, Petersson, S J, Schrøder, H D, Andersen, J L, Aagaard, P, Schjerling, P & Kjaer, M 2013, ' Ageing is associated with diminished muscle re-growth and myogenic precursor cell expansion early after immobility-induced atrophy in human skeletal muscle ', Journal of Physiology, vol. 591, no. 15, pp. 3789-3804 . https://doi.org/10.1113/jphysiol.2013.257121 |
| DOI: | 10.1113/jphysiol.2013.257121 |
| Popis: | Recovery of skeletal muscle mass from immobilisation-induced atrophy is faster in young than older individuals, yet the cellular mechanisms remain unknown. We examined the cellular and molecular regulation of muscle recovery in young and older human subjects subsequent to 2 weeks of immobility-induced muscle atrophy. Retraining consisted of 4 weeks of supervised resistive exercise in 9 older (OM: mean age) 67.3, range 61-74 yrs) and 11 young (YM: mean age 24.4, range 21-30 yrs) males. Measures of myofibre area (MFA), Pax7-positive satellite cells (SCs) associated with type I and type II muscle fibres, as well as gene expression analysis of key growth and transcription factors associated with local skeletal muscle milieu, were performed after 2 weeks immobility (Imm) and following 3 days (+3d) and 4 weeks (+4wks) of retraining. OM demonstrated no detectable gains in MFA (vastus lateralis muscle) and no increases in number of Pax7-positive SCs following 4wks retraining, whereas YM increased their MFA (P < 0.05), number of Pax7-positive cells, and had more Pax7-positive cells per type II fibre than OM at +3d and +4wks (P < 0.05). No age-related differences were observed in mRNA expression of IGF-1Ea, MGF, MyoD1 and HGF with retraining, whereas myostatin expression levels were more down-regulated in YM compared to OM at +3d (P < 0.05). In conclusion, the diminished muscle re-growth after immobilisation in elderly humans was associated with a lesser response in satellite cell proliferation in combination with an age-specific regulation of myostatin. In contrast, expression of local growth factors did not seem to explain the age-related difference in muscle mass recovery. |
| Databáze: | OpenAIRE |
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