Clinical and Experimental Immunology
| Autor: | Brodskyn, Claudia Ida, Beverley, S. M., Titus, R. G. |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1046/j.1365-2249.2000.01122.x |
| Popis: | Texto completo. Acesso restrito. p. 299-304 Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-30T17:10:30Z No. of bitstreams: 1 22222222222222222.pdf: 170228 bytes, checksum: 0c51c356e4df61ea77e5d3b8677bc3d5 (MD5) Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-08-05T13:12:28Z (GMT) No. of bitstreams: 1 22222222222222222.pdf: 170228 bytes, checksum: 0c51c356e4df61ea77e5d3b8677bc3d5 (MD5) Made available in DSpace on 2013-08-05T13:12:28Z (GMT). No. of bitstreams: 1 22222222222222222.pdf: 170228 bytes, checksum: 0c51c356e4df61ea77e5d3b8677bc3d5 (MD5) Previous issue date: 2000 In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-tsÿ) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania-infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-tsÿ did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the ®rst stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect. Salvador |
| Databáze: | OpenAIRE |
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