Usefulness of Mycobacterium tuberculosis molecular typing in a tuberculosis low-endemic agro-industrial setting of Brazil
| Autor: | Malaspina, Ana Carolina [UNESP], Cavalcanti, Hebe Rodrigues, Leite, Clarice Queico Fujimura [UNESP], Machado, Silvia Maria Almeida, Viana, Brunilde Helena Jung, Silva, Rosangela Maria Gaspareto, Hage, Eduardo Ferraz, Figueiredo, Walter Machado, Marques, Elisabeth, Ferrazoli, Lucilaine, Arbex, Marcos, Lessi, Marcio, Fonseca, Leila S., Rigouts, Leen, Saad, Maria Helena Féres [UNESP] |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal do Rio de Janeiro (UFRJ), Instituto Oswaldo Cruz/Fiocruz, Américo Brasilienses, Institut of Tropical Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
industrial area
Male pyrazinamide Asia streptomycin retrospective study Oligonucleotides geographic origin strain identification bacterial transmission Humans human drug sensitivity Tuberculosis Pulmonary restriction fragment length polymorphism spoligotyping Public Housing bacterium isolate Incidence health care facility molecular typing Mycobacterium tuberculosis bacterial strain tuberculosis Africa DNA Transposable Elements community living Female Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:23:36Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:30:07Z : No. of bitstreams: 1 2-s2.0-45849084706.pdf: 56577 bytes, checksum: 05aec86200a65b32ec8565684534d283 (MD5) Made available in DSpace on 2014-05-27T11:23:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-02 To highlight the transmission and major phylogenetic clades of Mycobacterium tuberculosis, a retrospective study was carried out at two health facilities in a small agro-industrial area in São Paulo, Brazil, that has a low tuberculosis incidence rate. IS6110-RFLP and spoligotyping were performed on the isolates, with the former revealing that 31.3% (35/112) of strains were clustered. Epidemiological links were found in 16 of the 35 clustered patients and were associated with transmission among patients living in public housing. Spoligotyping grouped 62.8% of the strains. The T genetic family predominated among the isolates. Of interest is that five strains had a pattern characteristic of African or Asian origin (ST535), and two others were of the rare localized type ST1888 (BRA, VEN). In addition, three new types-1889, 1890, and 1891-were identified. Spoligotyping showed that some ST may be circulating to or from Brazil, and RFLP revealed ongoing transmission in inadequately ventilated public-housing buildings. This may point to a failure in tuberculosis control policy. Faculdade de Ciéncias Farmacêuticas Universidade Estadual Paulista, São Paulo Hospital Universitário Pedro Ernesto Universidade Federal do Rio de Janeiro, Rio de Janeiro Laboratório de Microbiologia Celular Instituto Oswaldo Cruz/Fiocruz, Av. Brasil 4365, Rio de Janeiro 21045-900 Serviço Especial de Saúde de Araraquara Américo Brasilienses, São Paulo Instituto Adolfo Lutz Américo Brasilienses, São Paulo Instituto de Microbiologia Universidade Federal do Rio de Janeiro, Rio de Janeiro Hospital Nestor Goulart Reis Américo Brasilienses, São Paulo Institut of Tropical Medicine, Antwerp Faculdade de Ciéncias Farmacêuticas Universidade Estadual Paulista, São Paulo |
| Databáze: | OpenAIRE |
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