Molecular and metagenomic diagnostic methods of anaplasmataceae agents in domestic felids (domiciled and errant) and opossums of Metropolitan Region of Rio de Janeiro

Autor: Guimar?es, Andresa
Přispěvatelé: Baldani, Cristiane Divan, Dumler, John Stephen, Carvalho, Fab?ola Marques de, Souza, Aline Moreira de, Machado, Carlos Henrique, Balthazar, Daniel de Almeida
Jazyk: portugalština
Rok vydání: 2017
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-04-05T17:52:54Z No. of bitstreams: 1 2017 - Andresa Guimar?es.pdf: 1801107 bytes, checksum: e4ad1dcf396d5aa7844e5fbab6a2a85a (MD5) Made available in DSpace on 2019-04-05T17:52:54Z (GMT). No. of bitstreams: 1 2017 - Andresa Guimar?es.pdf: 1801107 bytes, checksum: e4ad1dcf396d5aa7844e5fbab6a2a85a (MD5) Previous issue date: 2017-07-04 CAPES Anaplasmataceae agents are gram-negative bacteria that are found in leukocytes, platelets or erythrocytes, forming morulae, characteristic of their infection. The transmission of these agents to man and animals occurs mainly by arthropod vectors. The participation of domestic felines and wild reservoirs has not yet been defined, with few studies on hemoparasitosis affecting these animals. The present study had as objective to investigate the presence of infection by Anaplasmataceae family agents in domestic felines and possums (Didelphis sp.) of the state of Rio de Janeiro, using molecular methods. We sought to sequence the PCR positive samples for validation of the same and to compare them with those deposited in Genbank, as well as to develop new diagnostic methods aiming at fast and high yield results, such as "deep sequencing", used in metagenomic assays. The aim of this study was to determine which hematological changes (hemogram, hematological morphology and hemoparasite inclusions) are more frequent and which associated factors are related to these in two distinct cat populations in the Metropolitan Region of Rio de Janeiro. Two populations of domestic cats, wandering cats (n = 105) and domiciled cats (n = 178) from the Metropolitan region of Rio de Janeiro were studied. Also, were studied 37 opossums collected from nature for rehabilitation, these were tested for the same agents as cats. The diagnosis of Ehrlichia canis, E. chaffeensis and Anaplasma spp. was performed by Polymerase Chain Reaction (PCR). In the comparison between the two populations of cats, there were significant differences between them (p
Databáze: OpenAIRE
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