Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)
| Autor: | Silva, Ivana Fernandes da [UNESP], Lopes Baldin, Edson Luiz [UNESP], Specht, Alexandre, Sosa-Gomez, Daniel Ricardo, Roque-Specht, Vania Ferreira, Morando, Rafaela [UNESP], Paula-Moraes, Silvana Vieira de |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Univ Brasilia, Univ Florida |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1861-0307 |
| Popis: | Made available in DSpace on 2018-11-26T16:00:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) EMBRAPA SEG We performed molecular and biological characterizations of immature stages of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) using insects from different states in Brazil, and different host plants. Three laboratory colonies of H. armigera were established with insects from the states of Sao Paulo, Bahia, and Distrito Federal, that were collected from citrus, cotton, and corn, respectively. For each colony, microsatellites were used to assess genetic similarity among insects. The biology of the immature stages from each colony also was compared under laboratory conditions (25 +/- 1 degrees C, 70 +/- 10% RH, 14: 10 h [L: D] photoperiod). Microsatellite analysis revealed genetic variability in sampled specimens and that the grouping of individuals was independent of the geographic origin. The utility of an artificial diet was validated during the comparative biology experiment, and the ingredients of the artificial diet were analyzed for purity, particle size, and the presence and quantity of aggregates. The mean development times of the larvae and pre-pupae from the 3 locations were similar, and mean total development time (egg to adult) was not significantly different. Sao Paulo State Univ, Coll Agron Sci, Dept Crop Protect, BR-18610307 Botucatu, SP, Brazil Embrapa Cerrados, BR 020,Km 18,Caixa Postal 08223, BR-73310970 Planaltina, Distrito Federa, Brazil Embrapa Soja, Rodovia Carlos John Str,Caixa Postal 231, BR-86001970 Londrina, Parana, Brazil Univ Brasilia, Fac UnB Planaltina, Area Univ N 1,Vila Nossa Senhora Fatima, BR-73345010 Planaltina, Distrito Federa, Brazil Univ Florida, West Florida Res & Educ Ctr, 4253 Expt Rd,Hwy 182, Jay, FL 32565 USA Sao Paulo State Univ, Coll Agron Sci, Dept Crop Protect, BR-18610307 Botucatu, SP, Brazil CNPq: 305649/2013-2 CNPq: 308947/2014-2 CNPq: 403376/2013-0 CNPq: 476691/2013-3 CNPq: 473041/2013-8 EMBRAPA SEG: 02.13.14.006 |
| Databáze: | OpenAIRE |
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