Investigação do efeito cardioprotetor do extrato hidroalcoólico de própolis vermelha em ratos infartados por modelo simpatomimético

Autor: Clementino Neto, José
Přispěvatelé: Ribeiro, Êurica Adélia Nogueira, Moura, Maria Aline Fidelis Barros de, Campesatto, Eliane Aparecida
Jazyk: portugalština
Rok vydání: 2015
Předmět:
Zdroj: Repositório Institucional da Universidade Federal de Alagoas (UFAL)
Universidade Federal de Alagoas (UFAL)
instacron:UFAL
Popis: Propolis is a natural resinous substance produced by bees of species Apis mellifera, through a variety of plant sources and various parts of the plants. Its chemical composition is complex and diverse. The red propolis is a type of propolis found in the mangroves of Brazilian northeastern. Some biological activities are associated with red propolis, among them, antioxidant, antimicrobial and anti-inflammatory activities. The present study was designed to evaluate the cardioprotective effect of hydroalcoholic extract of red propolis from Alagoas (HAERP) against isoproterenol-induced myocardial infarction in rats. For chemical characterization of HAERP was performed high performance liquid chromatography coupled to ultraviolet detector (HPLC-UV). Male Wistar rats (200-300g) were divided into 5 groups (n = 5), (Control = 0.3 mL Saline p.o. for 30 days), (ISO = infarction by isoproterenol 85 mg/kg s.c. on 2 consecutive days), (HAERP 50, 75 and 150 mg/kg, respectively, orally for 30 days and underwent infarction with isoproterenol 85mg/kg s.c in 29 and 30 days of treatment). On the 31st day the animals were euthanized, serum was collected and incubated in biochemical kits for the evaluation of creatine kinase total fraction (CK-NAC) and MB fraction (CK-MB), lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT), cholesterol total (COL) and triglycerides (TRI) markers of tissue damage. For morphometric analysis, were measured the weight cardiac, the thickness ventricular of left and right, its variations and relative increases, and reason cardiac weight by body weight (WC, TLV, TRV and WC/WB). For postmortem analysis the hearts were sectioned yet submitted to colorimetric test of triphenyltetrazolium chloride 1% (TTC1%). The results were expressed as mean ± SEM, and analyzed statistically by ANOVA one-way followed by Newman-keuls. Considered significant when *p
Databáze: OpenAIRE
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