Biocidal activity and biofilm production in Corynebacterium striatum

Autor: Souza, Cassius de
Přispěvatelé: Guaraldi, Ana Luiza de Mattos, Moreira, Lilian de Oliveira, Pereira, José Augusto Adler, Bôas, Maria Helena Simões Villas
Jazyk: portugalština
Rok vydání: 2014
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UERJ
Universidade do Estado do Rio de Janeiro (UERJ)
instacron:UERJ
Popis: Submitted by Boris Flegr (boris@uerj.br) on 2021-01-07T18:32:00Z No. of bitstreams: 1 Cassius de Souza Dissertacao completa.pdf: 2534078 bytes, checksum: 1e97ddb0fc7f0f2f8c9268ab3ec65e73 (MD5) Made available in DSpace on 2021-01-07T18:32:00Z (GMT). No. of bitstreams: 1 Cassius de Souza Dissertacao completa.pdf: 2534078 bytes, checksum: 1e97ddb0fc7f0f2f8c9268ab3ec65e73 (MD5) Previous issue date: 2014-02-26 Corynebacterium striatum has been linked to a number of cases of nosocomial infections, sometimes fatal, mainly in immunocompromised patients that use invasive devices. The occurrence of outbreaks in nosocomial environments caused by clones multi-resistant to antimicrobial agents (MDR) may include C. striatum among pathogens responsible for infections related to the health service (IRAS). Until now, studies focused on the investigation of virulence factors that may contribute to IRAS caused by C. striatum were not found in the literature. Accordingly, we investigated the biofilm production on inert solid surfaces and the susceptibility to biocidal products by planktonic and sessile forms of 14 strains of C. striatum of pulsotypes I-IV that were isolated during an nosocomial outbreak in the metropolitan region of Rio de Janeiro. Micro-organisms showed the ability to adhere to abiotic, hydrophilic, hydrophobic and metallic surfaces at different intensities. The epidemic clone (PFGE I-MDR) demonstrated a greater capacity to adhere to glass, plastic and metal surfaces. The scanning electron microscopy assays confirmed the ability to produce mature biofilms on polyurethane catheters by C. striatum of pulsotypes I, II, III e IV. The susceptibility to biocides was assessed by the disk diffusion and Time Kill methods. Results of the disk-diffusion screening test showed zones of inhibition with reduced diameters for C. striatum strains that remained viable in the presence of biocides when evaluated by the Time Kill test. Susceptibility profiles of planktonic forms to glutaraldehyde 2%, peracetic acid 1%, Virkon 1%, sodium hypochlorite 1%, chlorhexidine 2%, benzalkonium chloride sodium sulphate 2%, ethyl alcohol 70% and iodized alcohol (70/1%) were assessed by the Time Kill test at incubation times of 5, 10, 15 and 30 minutes. C. striatum showed tolerance to biocides for reduced periods of time (0,5< t
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