Efecto fotodinámico de Azul de metileno y Azul de toluidina, sobre biofilm de Streptococcus mutans y Candida albicans
| Autor: | Alanya-Ricalde, José Antonio, Miranda-Barriga, Lugó, Llanos-Carazas, Mónica Yizely, Acurio-Medina, Sheila, Miranda-Solis, Franklin |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 14 No. 4 (2021): October-December; 459-465 Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 14 Núm. 4 (2021): Octubre-Diciembre; 459-465 Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo instacron:HNAAA |
| ISSN: | 2227-4731 2225-5109 |
| DOI: | 10.35434/rcmhnaaa.2021.144 |
| Popis: | Objetive: To determine the photodynamic effect of methylene blue (MB) and toluidine blue (TBO) activated with therapeutic laser on Streptococcus mutans and Candida albicans biofilms. Material and methods: Sixty-one biofilm samples were analyzed in brain heart culture media (BHI) for 6 days, the samples were divided into 4 groups and immersed in: experimental group in 0.01% MB and 0.05% TBO, positive control group in 0.12% chlorhexidine gluconate (CHX) and negative control group in 0.9% sodium chloride (NaCl). The MB and TBO samples were irradiated with therapeutic laser with a wavelength of 660 nm, and 9 J/cm2 of power, each enamel block was in post-irradiation culture. The samples were cultured separately for the S. mutans and C. albicans strains, the method used was that of serial dilutions for the quantification of CFU after 5 days of culture. For the analysis, Student's T and Anova of one factor were used. Results: finding statistical differences between the four experimental groups in microbial growth (ANOVA p |
| Databáze: | OpenAIRE |
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