Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
| Autor: | Elia, E.M., Quintana, R., Carrere, C., Bazzano, M.V., Rey-Valzacchi, G., Paz, D.A., Pustovrh, M.C. |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
nonhuman
ovary follicle nitric oxide synthase vasculotropin animal model animal experiment article progesterone Evans blue enzyme linked immunosorbent assay Western blotting body weight blood vessel permeability female ovary hyperstimulation priority journal estradiol immunohistochemistry controlled study rat cyclooxygenase 2 metformin protein expression pathophysiology |
| Zdroj: | J. Ovarian Res. 2013;6(1) Biblioteca Digital (UBA-FCEN) Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
| Popis: | Background: In assisted reproduction cycles, gonadotropins are administered to obtain a greater number of oocytes. A majority of patients do not have an adverse response; however, approximately 3-6% develop ovarian hyperstimulation syndrome (OHSS). Metformin reduces the risk of OHSS but little is known about the possible effects and mechanisms of action involved. Objective. To evaluate whether metformin attenuates some of the ovarian adverse effects caused by OHSS and to study the mechanisms involved. Material and methods. A rat OHSS model was used to investigate the effects of metformin administration. Ovarian histology and follicle counting were performed in ovarian sections stained with Masson trichrome. Vascular permeability was measured by the release of intravenously injected Evans Blue dye (EB). VEGF levels were measured by commercially immunosorbent assay kit. COX-2 protein expression was evaluated by western blot and NOS levels were analyses by immunohistochemistry. Results: Animals of the OHSS group showed similar physiopathology characteristics to the human syndrome: increased body weight, elevated progesterone and estradiol levels (P |
| Databáze: | OpenAIRE |
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