Effects of shear rate and oxygen transfer in the conventional bioreactor on the production of clavulanic acid by Streptomyces clavuligerus

Autor: Ribeiro, Renata Maria de Magalhães Gomes Pontes
Přispěvatelé: Universidade Estadual Paulista (Unesp), Cerri, Marcel Otavio
Jazyk: portugalština
Rok vydání: 2017
Předmět:
Zdroj: Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Submitted by Renata Maria De Magalhães Gomes Pontes Ribeiro Marinho (gribeiro.renata@gmail.com) on 2020-05-26T14:03:00Z No. of bitstreams: 1 Dissertação_FINAL_RENATA MARIA_M-G-P_RIBEIRO_CORRIGIDO repositorio maio 2020.pdf: 2687198 bytes, checksum: 9aafdb74209392c8cd6b3a9402b349f7 (MD5) Submitted by Renata Maria De Magalhães Gomes Pontes Ribeiro Marinho (gribeiro.renata@gmail.com) on 2020-05-26T14:03:00Z No. of bitstreams: 1 Dissertação_FINAL_RENATA MARIA_M-G-P_RIBEIRO_CORRIGIDO repositorio maio 2020.pdf: 2687198 bytes, checksum: 9aafdb74209392c8cd6b3a9402b349f7 (MD5) Submitted by Renata Maria De Magalhães Gomes Pontes Ribeiro Marinho (gribeiro.renata@gmail.com) on 2020-05-26T14:03:00Z No. of bitstreams: 1 Dissertação_FINAL_RENATA MARIA_M-G-P_RIBEIRO_CORRIGIDO repositorio maio 2020.pdf: 2687198 bytes, checksum: 9aafdb74209392c8cd6b3a9402b349f7 (MD5) Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Submitted by Renata Maria De Magalhães Gomes Pontes Ribeiro Marinho (gribeiro.renata@gmail.com) on 2020-05-26T14:03:00Z No. of bitstreams: 1 Dissertação_FINAL_RENATA MARIA_M-G-P_RIBEIRO_CORRIGIDO repositorio maio 2020.pdf: 2687198 bytes, checksum: 9aafdb74209392c8cd6b3a9402b349f7 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Submitted by Renata Maria De Magalhães Gomes Pontes Ribeiro Marinho (gribeiro.renata@gmail.com) on 2020-05-26T14:03:00Z No. of bitstreams: 1 Dissertação_FINAL_RENATA MARIA_M-G-P_RIBEIRO_CORRIGIDO repositorio maio 2020.pdf: 2687198 bytes, checksum: 9aafdb74209392c8cd6b3a9402b349f7 (MD5) Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Submitted by Renata Maria De Magalhães Gomes Pontes Ribeiro Marinho (gribeiro.renata@gmail.com) on 2020-05-26T14:03:00Z No. of bitstreams: 1 Dissertação_FINAL_RENATA MARIA_M-G-P_RIBEIRO_CORRIGIDO repositorio maio 2020.pdf: 2687198 bytes, checksum: 9aafdb74209392c8cd6b3a9402b349f7 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Approved for entry into archive by Maria Irani Coito (irani@fcfar.unesp.br) on 2020-05-27T23:52:03Z (GMT) No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Made available in DSpace on 2020-05-27T23:52:03Z (GMT). No. of bitstreams: 1 ribeiro_rmmgp_me_arafcf_int.pdf: 3451853 bytes, checksum: 57e5b8704ce2bf537b7cffe146fedd96 (MD5) Previous issue date: 2017-04-24 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Streptomyces clavuligerus é uma bactéria filamentosa, capaz de produzir ácido clavulânico (AC), um importante fármaco β-lactâmico. Sendo necessário o controle de parâmetros como pH, temperatura, homogeneização do caldo, adequada transferência de oxigênio e cisalhamento para melhora de processos biotecnológicos. Sabe-se que a partir da transferência de oxigênio e da velocidade média de cisalhamento imposta ao sistema nesses biorreatores, por meio do uso de maiores ou menores rotações dos impelidores, a produção de ácido clavulânico é afetada, pois essas variáveis influenciam no crescimento do microrganismo, na reologia e morfologia do microrganismo presente no caldo fermentativo e na formação e estabilidade da molécula de AC. No presente trabalho, foram realizados experimentos para determinação volumétrica de oxigênio com diferentes conformações para encontrar o melhor posicionamento dos impelidores. Com a melhor conformação, estudou- se a transferência de oxigênio com diferentes vazões e rotações de ar. Os resultados desses experimentos mostram que um sistema pode apresentar melhores ou piores resultados de transferência de oxigênio para o processo, dependendo da sua geometria. A variação na distância entre os impelidores aumentou em até 83% a transferência de oxigênio do biorreator. Foram realizados cultivos de Streptomyces clavuligerus em biorreator convencional com diferentes vazões, rotações e composição de gás para comparar os diversos efeitos sofridos na morfologia dos microrganismos, consumo de substrato, densidade ótica e reologia e produção de ácido clavulânico. Com diferentes rotações e aeração foi possível estudar a velocidade de cisalhamento e transferência de oxigênio independentemente, sendo capaz de saber como cada variável afeta a produção de ácido clavulânico. O aumento da transferência de oxigênio acelerou a produção do ácido clavulânico, mas não aumentou sua produção, os resultados obtidos evidenciam que a condição de cisalhamento é a grande responsável pelo aumento na produção do fármaco, pois ocorre a formação de hifas não ramificadas no caldo fermentativo, aumentando a produção do metabólito em até 45%. Streptomyces clavuligerus is a filamentous bacterium, producing of clavulanic acid (CA), an important β-lactam drug. It is necessary to control parameters such as pH, temperature, broth homogenization, adequate oxygen transfer and shear to improve biotechnological processes. Knows that from the transfer of oxygen and the average shear velocity imposed to the system in these bioreactors, through the use of greater or smaller rotations of the impellers, affect the production of clavulanic acid, since these variables influence the growth of the microorganism, in the rheology and morphology of the microorganism present in the fermentative broth and in the formation and stability of the CA molecule. In the present work, experiments were carried out for the volumetric determination of oxygen with different conformations to find the best positioning of the impellers. With the best conformation, the transfer of oxygen with different flow rates and air rotations was studied. The results of these experiments show that a system can present better or worse oxygen transfer results to the process depending on its geometry. The variation in distance between the impellers increased by up to 83% the transfer of oxygen from the bioreactor. Streptomyces clavuligerus cultures were grown in conventional bioreactor with different flow rates, rotations and gas composition to compare the different effects of microorganism’s morphology, substrate consumption, optical density and rheology and clavulanic acid production. With different rotations and aeration it was possible to study the shear rate and oxygen transfer independently, being able to know how each variable affects the production of clavulanic acid. The increase in oxygen transfer accelerated the production of clavulanic acid, but did not increase its production, the results obtained show that the shear condition is the major responsible for the increase in the production of the drug, since the formation of unbranched hyphae occurs in the fermentative broth , Increasing the production of the metabolite by up to 45%. CAPES: 1480496 001
Databáze: OpenAIRE
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