Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase

Autor:	Fragoso, Rodrigo da Rocha, Lourenço, Isabela Tristan, Batista, João Aguiar Nogueira, Oliveira-Neto, Osmundo Brilhante, Silva, Maria Cristina Mattar, Rocha, Thales Lima, Coutinho, Marise Ventura, Grossi-de-Sa, Maria Fátima
Jazyk:	angličtina
Rok vydání:	2009
Předmět:	RT-PCR- reverse transcription and polymerase chain reaction Meloidogyne RACE- rapid amplification EST expressed sequence tag CN-cyst nematode AP- aspartic proteinase L2 - second-stage larva or juvenile RKM-Root-knot nematode cDNA cloning Aspartic proteinase ORF- open read frame Cathepsin D Nematode
Zdroj:	Repositório Institucional da UCB Universidade Católica de Brasília (UCB) instacron:UCB
Popis:	Made available in DSpace on 2016-10-10T03:52:21Z (GMT). No. of bitstreams: 5 Meloidogyne incognita_molecular cloning and characterization.PDF: 1058825 bytes, checksum: 149f0214de61c9c6736076381cd2c9e5 (MD5) license_url: 52 bytes, checksum: 2f32edb9c19a57e928372a33fd08dba5 (MD5) license_text: 24259 bytes, checksum: f1f24f769b03eb8f9cd3f53c1090841c (MD5) license_rdf: 24658 bytes, checksum: 9d3847733d3c0b59c7c89a1d40d3d240 (MD5) license.txt: 1887 bytes, checksum: 445d1980f282ec865917de35a4c622f6 (MD5) Previous issue date: 2009 Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second- stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank ™ (DQ360827) sequences showed 59–71% identity with nematode- specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control. Sim Publicado
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______3056::e36db8285a3c6a920c946147b9e57d07 http://twingo.ucb.br:8080/jspui/handle/10869/482 Zobrazit plný text záznamu