Estudo fitoqu??mico dos extratos metan??licos de flores e folhas de Macrolobium acaciifolium e algumas avalia????es biol??gicas dos seus extratos

Autor: Silva, David Ribeiro da
Přispěvatelé: Nunez, Cec??lia Ver??nica, Nunomura, Rita de Cassia Saraiva, Albuquerque, Patricia Melchionna
Jazyk: portugalština
Rok vydání: 2018
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by David Ribeiro da Silva (david.ribeiro07@gmail.com) on 2020-01-07T19:42:13Z No. of bitstreams: 3 55-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5446743 bytes, checksum: 20794be2cafce7e86abe7398fe04419a (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Rejected by PPGQ Qu??mica (ppgqsecretaria@gmail.com), reason: Favor deixar na capa da disserta????o apenas a logomarca da UFAM e retirar as demais. on 2020-01-07T22:38:10Z (GMT) Submitted by David Ribeiro da Silva (david.ribeiro07@gmail.com) on 2020-01-08T13:06:57Z No. of bitstreams: 3 56-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5421619 bytes, checksum: 1cf61258edcf275c07c22235f4bbc11a (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Approved for entry into archive by PPGQ Qu??mica (ppgqsecretaria@gmail.com) on 2020-01-08T13:24:56Z (GMT) No. of bitstreams: 3 56-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5421619 bytes, checksum: 1cf61258edcf275c07c22235f4bbc11a (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Rejected by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br), reason: Incluir ficha catalogr??fica na disserta????o, ap??s a folha de rosto. Voc?? pode elabor??-la automaticamente em: https://biblioteca.ufam.edu.br/servicos/elaboracao-de-ficha-catalografica on 2020-01-08T13:41:07Z (GMT) Submitted by David Ribeiro da Silva (david.ribeiro07@gmail.com) on 2020-01-08T14:37:43Z No. of bitstreams: 3 57-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5515692 bytes, checksum: 0e3351503fcd72bb026a35939765f1ae (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Approved for entry into archive by PPGQ Qu??mica (ppgqsecretaria@gmail.com) on 2020-01-08T14:56:23Z (GMT) No. of bitstreams: 3 57-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5515692 bytes, checksum: 0e3351503fcd72bb026a35939765f1ae (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2020-01-08T18:15:30Z (GMT) No. of bitstreams: 3 57-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5515692 bytes, checksum: 0e3351503fcd72bb026a35939765f1ae (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Made available in DSpace on 2020-01-08T18:15:30Z (GMT). No. of bitstreams: 3 57-DEFESA-DAVID RIBEIRO DA SILVA - VERS??O IMPRESS??O.pdf: 5515692 bytes, checksum: 0e3351503fcd72bb026a35939765f1ae (MD5) Carta de Encaminhamento autodep??sito.pdf: 333206 bytes, checksum: 971e103579bee2054c32e156e90c03e8 (MD5) Folha de aprova????o.pdf: 307290 bytes, checksum: afe83555df7222c8a772a872d195e0e8 (MD5) Previous issue date: 2018-02-15 FAPEAM - Funda????o de Amparo ?? Pesquisa do Estado do Amazonas Many species of plants and trees have not yet been studied and require and chemical biological data for verification of its constituents and activities, seeking the discovery of new molecules and ways of using them in health benefits. The Amazon contains many species not studied and Macrolobium acaciifolium is one of them, which is a tree of the Caesalpinioidea (Fabaceae) subfamily and is present not only in the Amazon, but in other states of Brazil, as well as Central and South American countries. The objective of this work was to study chemically the secondary metabolites of M.acaciifolium and to test the antioxidant, toxic and antibacterial potential of this extracts. Hexanic and methanolic extracts were obtained by cold maceration with the aid of ultrasound and its potential antioxidants were evaluated by the DPPH radical and the Fe3+/Phenantroline complex, as well as toxic tests against Artemia salina and some strains of bacteria. The phytochemical study of the methanolic extract of the flowers allowed the isolation of the flavonoid kaempferol-3-O-rutinoside and of the methanolic extract of the leaves to apigenin, luteolin-3???-O-??-L-rhamnoside and luteolin-4???-O-??-L-rhamnoside. The assays performed indicated that the leaves' methanolic extract has a high antioxidant potential and the leaf hexanic and methanolic extracts of the branches have high bacteriostatic potentials, but all were nontoxic against Artemia salina. Luteolin-4???-O-??-L-rhamnoside is not reported in the literature consulted and its presence, together with the flavonoids isolated, suggest the antioxidant potential of the leaf methanolic extract. Muitas esp??cies de plantas e ??rvores ainda n??o foram estudadas e necessitam de dados qu??micos e biol??gicos para verifica????o de seus constituintes e atividades, buscando a descoberta de mol??culas novas e meios de utiliz??-las em benef??cios da sa??de. A Amaz??nia cont??m muitas esp??cies n??o estudadas e Macrolobium acaciifolium ?? uma delas, a qual ?? uma ??rvore da sub-fam??lia Caesalpinioideae (Fabaceae) e est?? presente n??o apenas na Amaz??nia, mas em outros estados do Brasil, al??m de pa??ses da Am??rica Central e do Sul. O objetivo deste trabalho foi estudar quimicamente os metab??litos secund??rios dos extratos de M. acaciifolium e testar o potencial antioxidante, t??xico e antibacteriano destes extratos. Os extratos hex??nicos e metan??licos foram obtidos por macera????o a frio com o aux??lio do ultrassom e seus potenciais antioxidantes foram avaliados frente o radical DPPH e o complexo Fe3+/Fenantrolina, assim como testes t??xicos contra Artemia salina e algumas cepas de bact??rias. O estudo fitoqu??mico do extrato metan??lico das flores permitiu o isolamento do flavonoide kaempferol-3-rutinos??deo e do extrato metan??lico das folhas a apigenina, luteolina-3???-O-??-L-ramnos??deo e luteolina-4???-O-??-L-ramnos??deo. Os ensaios realizados indicaram que o extrato metan??lico das folhas possui elevado potencial antioxidante e os extratos hex??nico das folhas e metan??lico dos galhos possui elevados potenciais bacteriost??ticos, mas todos se mostraram n??o t??xicos contra Artemia salina. Luteolina-4???-O-??-L-ramnos??deo n??o possui relato na literatura consultada e a sua presen??a, junto com os outros flavonoides isolados, sugere o potencial antioxidante do extrato metan??lico das folhas. Muito intuitiva e totalmente compreens??vel. Subst??ncia luteolina-4???-O-??-L-ramnos??deo isolada e identificada pela primeira vez.
Databáze: OpenAIRE
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