Efecto de la Suplementación del Medio de Maduración con Cisteamina y de Dos Medios de Cultivo (KSOMaa y SOF) en la Fecundación in vitro de Ovocitos Bovinos
| Autor: | Quintanilla M., Lisbeth, Huanca L., Wilfredo, Córdova G., Alejandro, Ampuero B., Antonio, Benavides I., Lenin |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 3 (2015); 462-468 Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 3 (2015); 462-468 Revistas Universidad Nacional Mayor de San Marcos Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| ISSN: | 1682-3419 1609-9117 |
| Popis: | The effect of the cysteamine in maturation medium on the oocyte cleavage rate and the effect of culture media KSOMaa and SOF on in vitro embryo development rate at 8 days post-fertilization was evaluated. A total of 680 oocytes from cattle ovaries were randomly distributed in two treatments: T1 (n=321) maturation medium TCM-199 and T2 (n=359) maturation medium TCM-199 supplemented with 100 mM cysteamine. Oocytes were fertilized after 24 h with frozen semen from a single bull and cultured in KSOMaa for 18 h and the fertilization rate was assessed 72 h after division. In the second phase, after the first culture, both groups were cultivated using two culture media: KSOMaa and SOF until day 8 post-fertilization. The results of the first phase showed a cleavage rate of 43.6% for the control group and 46.0% for the cysteamine supplemented group, without difference between groups. In phase 2, the blastocyst rate was 18.0% for the TCM-199 + KSOMaa and 20.9% for TCM-199 with cysteamine + KSOMaa (p |
| Databáze: | OpenAIRE |
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